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The observed long time constant in the transient current trace is explained by the dielectric absorption of the membrane capacitor.īranton D, Deamer DW, Marziali A et al (2008) The potential and challenges of nanopore sequencing. As a result of the transient charging process, a non-constant driving force pulls the DNA back to nanopores faster than theoretically predicted. The COMSOL simulation reveals that the membrane capacitance plays an important role in determining the electric field distribution during the charging process. The prohibited time when the data acquisition system is saturated by the transient current is derived by equivalent circuit analysis and finite element simulation. Numerical calculation of molecular motion clearly shows the recapture dynamics with different delay times. We performed and analyzed the DNA translocation and recapture experiment in a silicon nitride solid-state nanopore. However, the transient current at the moment of voltage reversal prevents the observation of instantly recaptured molecules and invalidates the continuous DNA ping-pong control. Repeat recapture of a single DNA molecule reveals the dynamics in DNA translocation through a nanopore and may significantly increase the signal-to-noise ratio for DNA base distinguishing. Motion control of a single molecule through a solid-state nanopore offers a new perspective on detecting and analyzing single biomolecules.
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